Counting Chambers

Counting Chambers from Hausser

These chambers are the finest quality, optically ground, and polished milled glass chambers available. The chamber is diamond etched and has a double improved Neubauer Ruling, which has a worldwide reputation in hospitals and laboratories for unmatched reliability, meeting the most demanding of standards. The standard Hausser blood counting chambers are one piece construction (measuring 75mmx32mmx4.5mm) ensuring long term durability and absolute accuracy in measurement and count.

We offer both the standard (incline) and the new “V-Load” counting hambers for different charging methods. The tolerances are ( 2% of the volume). Cell Depth: 0.100 mm ( +/-2%); Volume: 0.1 Microliter Ruling; Pattern: Improved Neubauer, 1/400 Square mm Rulings cover 9 square millimeters. Boundary lines of the Neubauer rulings are the center lines of the groups of three. (These are indicated in the illustration ). The central square millimeter is ruled into 25 groups of 16 small squares, each group separated by triple lines, the middle one of which is the boundary. The ruled surface is 0.10 mm below the cover glass, so that the volume over each of 16

Made to Last

In addition to the standard ruling patterns which are etched permanently onto the polished surface, we offer the popular “Bright-Line” counting chamber. This bright-line counting chamber allows the ruling to appear brightly illuminated providing sharp contrast to the bright lines and cells, helping the user with accurate counts and less eyestrain. The triple lines show clearly which cells lie within the counting area and eliminate guesswork.We guarantee that all marks are permanent, and can not be removed even after repeated washings.

Other Chambers and Accessories

In addition to the blood counting chambers, we also offer a variety of other counting chambers and accessories which compliment our discipline.

• The Phase Hemacytometer: used for phase microscopy; it is a one piece brightlined hemacytometer with a reduced thickness.
• The Petroff Hausser Counting Chamber: used for bacteria counting.
• The Sperm Counting Chambers: used primarily in fertility testing; it is offered in a series of depths (10, 20, 40 microns).
• The Howard Mold Counter and the Sedgewick Rafter Counting Chamber are also available as well as a series of hemacytometer and counting chamber cover glasses.

Double Neubauer Ruled Metallized Counting Chamber

An “H” shape moat which forms 2 counting areas are metal plated and double Neubauer ruled providing sharp 

bright line contrast for consistent cell counts. A “V” cut on each area is used to control the possibility of overflow. Triple dividing lines clearly show cells within the counting area. The ruled surface is 0.1mm below the cover glass; liquid capacity is 0.1mm3/mm2 and 0.00025mm3/grid square.

Leica Bright-Line Counting Chamber

Designed by Leica for accurate counting of blood cells platelets, dust/yeast cluster and specimen samples. 

Sharp contrast from rhodium plated on “V-Load”. Triple dividing lines clearly delineate counting area for accurate and precise results. Molded from a single piece of thermal and shock resistant glass with a polished chamber and cover glass assuring precise capillary loading and even cell distribution. Fluid volume is 0.1 mm3/mm2 and 0.00025mm2 per each square.

Cellometer™- The Disposable Cell Counting Chamber!!

Cell Counting Made Easy

These Disposable Cell Counters are an enclosed chamber with two ports for sample introduction. The chamber contains precisely spaced lines in a grid pattern. There are two counting chambers per slide for replicates The counting grid pattern is the Improved Neubauer, as in a common Hemacytometer. The consistent pattern design allows the standard cell counting procedure to be followed.

With a pipetor, 15 to 20 microlilter cell-containing sample is introduced into the counting chamber through one of the ports. Liquid spreads inside the chamber, replaces air, which escapes through the opposing port.

The cell counting chamber is placed under a phase contrast optical microscope with a typical magnification of 100. The microscope is focused both onto the grid pattern and the cell particles. Standard protocols are followed for cell counting.

1. Count the total number of cells found in 4 large corner squares (C_total)
2. Cell concentration = Dilution x C_total / Volume

Let the Cellometer™ replace your Hemacytometers, which are traditionally used for cell. Counting!!

Benefits of the Cellometer™:

1. No washing after counting. Extremely important for people whom work with infectious samples.
2. No need to place the cover slide
3. Easy sample introduction. By integrating the cover slide into the counting chamber, the Cellometer™ eliminates the mistakes that may be caused by misplacement of the cover slide or misleading of the sample- two major sources for counting errors when using the Hemacytometer.
4. Made from plastic. Ideal for BioSafety level 3.

Technical Data

Comparisons of Counting Protocols

Step	Cellometer™	Hemacytometer
1	Mix cell suspension	Mix cell suspension
2	Pipette 15 to 20 microliter into sample port	Carefully load with 10 microliter solution
3	Count cells in 4 corner squares (C_total)	Count cells in 4 corner squares (C_total)
4	Cell Concentration = Dilution x (C_total / 4.0 ) x10 4	Cell Concentration = Dilution x (C_total / 4.0 ) x10 4
5	Dispose used Cellometer	Wash and dry cover slide and base

Description of the experiment:

1. Cell Type: Human T lymphocyte
2. Counting Devices: Cellometer™ with glass base (CG2), all plastic Cellometer™ (CP2) and a standard bright line Hemacytometer.
3. Cell counting performed alternating between Cellometer™ and Hemacytometer.
4. Statistical data analysis using Two-Sample T test.

Results:

	CP2-test 1	CP2-test 2	CG2
# of test (Cellometer™)	15	19	17
# of test (Hemacytometer)	8	17	17
Mean (Cellometer™)	1.78 x 10 5	1.83 x 10 5	1.87 x 10 5
Mean (Hemacytometer)	1.85 x 10 5	1.82 x 10 5	1.82 x 10 5
p-value	0.325	0.908	0.505

Conclusions:

1. Cell counts obtained using Cellometer™ and Hemacytometer  are consistent.
2. With Cellometer™, sample loading is simple and consistent.
3. There is no washing and drying after using Cellometer™, which  Eliminates liquid waste and Saves time and effort

source : Electron Microscopy Sciences